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Journal of Visualized Experiments (JoVE) is a video journal for biological research. |

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Testing the Physiological Barriers to Viral Transmission in Aphids Using Microinjection Cecilia Tamborindeguy1, Stewart Gray1, Georg Jander2 1Plant Pathology, Cornell University, 2Boyce Thompson Institute for Plant Research, Cornell University
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| Aphids are effective transmitters of plant viruses. Aphid microinjection of virus, the procedure we will show you today, is a technique allowing researchers to inject virus directly into the hemocoel of the aphid, bypassing the gut, one of the 2 major barriers for virus transmission in a circulative manner. The same technique is also used to inject dsRNA for RNAi.
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Microfabrication of chip-sized scaffolds for three-dimensional cell cultivation Stefan Giselbrecht1, Eric Gottwald1, Roman Truckenmueller2, Christina Trautmann3, Alexander Welle1, Andreas Guber4, Volker Saile4, Thomas Gietzelt5, Karl-Friedrich Weibezahn1 1Institute for Biological Interfaces, Karlsruhe Research Centre, 2Institute for BioMedical Technology, University of Twente, 3Department of Materials Research, Institute for Heavy Ion Research, 4Institute of Microstructure Technology, Karlsruhe Research Centre, 5Institute for Micro Process Engineering, Karlsruhe Research Centre
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| We present two processes for the microfabrication of porous polymer chips for three-dimensional cell cultivation. The first one is hot embossing combined with a solvent vapour welding process. The second one uses a recently developed microthermoforming process combined with ion track technology leading to a significant simplification of manufacture.
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Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures Maya Sieber-Blum1, 2, Yaofei Hu2 1Institute of Human Genetics and Northeast England Stem Cell Institute, Newcastle University, 2Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin
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| Here we show our method to isolate mouse epidermal neural crest stem cells (EPI-NCSC). Technique involves micro-dissecting whisker follicles, isolating the bulge and placeing it into tissue culture. EPI-NCSC start to emigrate from bulge explants onto the substratum within 3 - 4 days.
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