Journal of Visualized Experiments (JoVE) is a video journal for biological research. |
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02/08/2010
Andrew D. Klocko, Kaleena M. Crafton, Brian W. Walsh, Justin S. Lenhart, Lyle A. Simmons
Department of Molecular, Cellular, and Developmental Biology, University of Michigan-Ann Arbor
A detailed protocol is described for imaging the real time formation of DNA repair complexes in Bacillus subtilis cells.
02/05/2010
Bonnie N. Dittel
BloodCenter of Wisconsin, Blood Research Institute
To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.
02/04/2010
Mark Parker1, 2, 3, Aurore Brugeaud1, 2, Albert S. B. Edge1, 2, 4
1Department of Otology and Laryngology, Harvard Medical School, 2Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, 3Department of Communication Sciences and Disorders, Emerson College, 4Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology, Harvard and MIT
This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.
02/03/2010
Erica Andersen1, 2, 3, Namrata Asuri1, 2, 3, Matthew Clay2, 3, 4, Mary Halloran1, 2, 3, 4
1Genetics Training Program, University of Wisconsin-Madison, 2Department of Anatomy, University of Wisconsin-Madison, 3Department of Zoology, University of Wisconsin-Madison, 4Cell and Molecular Biology Training Program, University of Wisconsin-Madison
This protocol describes imaging of individual neurons or neural crest cells in living zebrafish embryos. This method is used to examine cellular behaviors and actin localization using fluorescence confocal time-lapse microscopy.
02/02/2010
Su-Qi Zou, Wu Yin, Ming-Jing Zhang, Chun-Rui Hu, Yu-Bin Huang, Bing Hu
Laboratory of Neurodevelopment and Repair, Department of Neurobiology and Biophysics, School of Life, University of Science and Technology of China
Optokinetic response has been widely used to assess the visual functions of larval zebrafish. Nevertheless, the standard protocol for larval fish is not yet readily applicable in adults 1-5. Here, we introduce how to measure the OKR of adult zebrafish using a new protocol which is established in our lab.
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